A schematic of the stopped-flow mixing experiment to probe salt-induced disassembly of nucleosome core particles. The left side shows NCPs in solution before the addition of sucrose. On the right, sucrose is added to increase solvent density, effectively erasing the signal from the protein. Pollack lab Biophysics is a science of shapes – the shapes of molecules like DNA as they wrap and unwrap around protein cores, for instance. Cornell researchers have unveiled a new method for observing such processes in real time. Professor of applied and engineering physics Lois Pollack and her research group have devised a way to watch dynamic movements and shape changes of molecules in solution using a new X-ray scattering method. In aNucleic Acids Researchpaper recently published online, they proved their method by observing transient nucleosome structures as DNA unwound from them. The work was done in collaboration with professor Lisa Gloss and her research group at Washington State University. When stored away, DNA is wrapped like thread around a core of eight proteins called histones. This assembly is known as a nucleosome core particle (NCP). The entire system takes many shapes as the DNA unwraps for processes including transcription and replication of genetic
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